TABLE 1.
Comparison of alpha and beta interferon pathway gene expression levels in infant and adult airway epithelial cell culturesa
| Gene | Infection | Avg CT (SD) |
Fold regulation | P value | |
|---|---|---|---|---|---|
| Infants | Adults | ||||
| MAL | − | 28.05 (1.1) | 25.44 (1.1) | −5.94 | 0.03 |
| IFNAR2 | − | 27.44 (0.6) | 28.14 (0.4) | 1.67 | 0.03 |
| CD70 | − | 27.07 (0.4) | 27.64 (0.5) | 1.53 | 0.05 |
| CIITA | + | 32 (0.44) | 30.13 (1.0) | −3.57 | 0.05 |
| CASP1 | + | 25.47 (0.67) | 24.26 (0.52) | −2.27 | 0.02 |
| RPL13A | + | 29.19 (0.42) | 28.07 (1.1) | −2.17 | 0.05 |
| PTTG1 | + | 21.96 (0.53) | 23.17 (0.90) | 2.34 | 0.02 |
Shown are significantly regulated genes from an RT2 profiler PCR array for alpha and beta interferons comparing infant and adult cultures. − indicates mock-infected cultures, and + indicates H1N1 at an MOI of 1 at 24 h postinfection. Also shown are average cycle threshold (CT) values, with standard deviations in parentheses (n = 4 per group). Fold regulation represents fold change values, with values of <1 indicating a negative number. Fold changes in infant gene expression levels over adult levels were calculated as 2−[(delta CT infant) − (delta CT adult)], where delta CT is the CT value of the gene of interest minus the mean CT value of housekeeping genes. The P value was determined based on a two-tailed t test of replicate 2(−delta CT) values.