TABLE 1.
Serine/threonine kinases that exhibited efficient phosphorylation of NS5A
| Kinase | LU | Relative kinase activitya |
|
|---|---|---|---|
| FL | D3 | ||
| TSSK2 | 8,310 | 4.24 | 14.94 |
| CKII-α′ | 5,068 | 2.30 | 8.20 |
| Plk1 | 3,230 | 2.70 | 44.65 |
| CKI-γ2 | 2,602 | 4.31 | 3.15 |
| CKI-γ1 | 2,560 | NA | 138.69 |
| CKI-γ3 | 2,218 | 0.23 | 52.51 |
| CKI-ε | 2,012 | 7.95 | 9.48 |
| PKACβ | 1,854 | 0.15 | 69.54 |
| CKI-α | 1,354 | 4.66 | 1.00 |
a
LU, light units from the AlphaScreen; FL, full-length NS5A; D3, domain III of NS5A. NA, not assessed due to overlap between purified kinases and NS5A on the gel. The relative kinase activity is the fold increase of the in vitro activity of each kinase relative to that of DHFR.