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Interactions between ICP22, UL31, UL34, UL47, and Us3 in HSV-1-infected cells. HEL cells infected with wild-type HSV-1(F) (A to C), YK423 (MEF-ICP22) (A), YK539 (MEF-UL31) (B), or YK538 (MEF-UL34) (C) at an MOI of 5 for 18 h were harvested, immunoprecipitated (IP) with anti-Myc antibody (α-Myc), and analyzed by immunoblotting (IB) with the antibodies indicated. WCE, whole-cell extract.
