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Effect of Us3 kinase activity on localization of ICP22 and UL34 in HSV-1-infected cells. HEL cells infected with wild-type HSV-1(F), YK511 (Us3K220M), or YK513 (Us3K220M-repair) at an MOI of 5 for 13 h were fixed, permeabilized, stained with anti-ICP22 and anti-UL34 antibodies, and examined by confocal microscopy.
