TABLE 2.
Effect of the ICP22-null mutation on distribution of virus particles in infected Vero cells
| Virus | Avg % of virus particles in morphogenetic stage ± SE (no. of virus particles) |
Total no. of particles counted/total no. of cells | ||||
|---|---|---|---|---|---|---|
| Nucleocapsids in the nucleus | Enveloped virions in the perinuclear space | Nucleocapsids in the cytoplasm | Enveloped virions in the cytoplasm | Extracellular enveloped virions | ||
| HSV-1(F) | 42.0 ± 2.9a (1,206) | 16.6 ± 3.0a (516) | 10.7 ± 2.0 (279) | 11.1 ± 1.6 (323) | 19.7 ± 3.7 (539) | 2,863/20 |
| YK421 (ΔICP22) | 60.4 ± 4.1 (1,367) | 7.1 ± 0.9 (140) | 4.6 ± 0.9 (97) | 7.6 ± 1.8 (188) | 20.3 ± 3.2 (391) | 2,183/20 |
| YK422 (ΔICP22-repair) | 47.2 ± 4.3a (871) | 14.8 ± 3.4a (316) | 6.6 ± 1.5 (140) | 10.3 ± 2.4 (208) | 21.0 ± 2.6 (387) | 1,922/20 |
a
Statistically significant difference from YK421 (ΔICP22) (P < 0.05).