FIG 5.

Cell type-dependent neutralization can be eradicated by expressing DC-SIGNR on Vero cells. The role of lectin-mediated attachment in the cell type-dependent neutralization of WNV was explored using a Vero cell line that was engineered to express DC-SIGNR (Vero-DC-SIGNR). (A) The specific infectivities of WNV-Furin and WNV-prM were measured on Vero, Vero-DC-SIGNR (VeroR), and Raji-DC-SIGNR (RajiR) cells. The ratio of the specific infectivity of WNV-prM to WNV-Furin virus was calculated for each cell type. Error bars display the standard errors from three experiments performed using independent stocks of viruses. Statistical comparisons were performed using a paired t test. (B and C) Neutralization of WNV-Std on the three cell types was evaluated using MAbs E16 (B) and E53 (C). Error bars display the range of the data from duplicate measurements. Data are representative of three independent experiments; statistical comparisons were performed using a paired t test.