FIG 5.
Protection from CVB3-associated pathology is independent of the delivery route. CAR WT and ncKO animals were inoculated intravenously with 1 × 106 PFU of the CVB3 Nancy strain. (A) At 10 dpi, viral RNA was present in heart and pancreas of WT but not ncKO animals as detected by in situ hybridization of paraffin sections. (B) CVB3-specific antibodies were detected in both WT and ncKO animals by using a coxsackievirus IgG-specific ELISA. The immune response was significantly reduced in ncKO animals. (C) Serum levels of RANTES at 10 dpi were increased only in WT animals. *, P < 0.05; ***, P < 0.001. WT, n = 4; ncKO, n = 5. Bar, 50 μm.