FIG 4.

Western immunoblots of UL20 protein (FLAG epitope) in lysates from cells infected with the indicated viruses. Confluent monolayers of Vero cells were infected with the viruses at an MOI/cell of 2. At 24 h.p.i., cell lysates were collected, run on SDS-PAGE gels, and probed with anti-FLAG antibody. Lanes: 1, Vero cell lysate; 2, parental wild-type lysate; 3, UL20Δ6 lysate; 4, UL20ΔPhe lysate; 5, UL20F222A lysate; 6, UL20F210A lysate; 7, UL20F205-206A lysate. Molecular weights (in thousands) are shown at the left.