FIG 8.

Proximity ligation assay to determine UL20p and UL37 interactions. Vero cells were infected with viruses at a high MOI/cell of 10, and PLA was performed at 18 h.p.i. (a to i) Negative-control mock infection (a), negative control with HSV-1(F) strain infection (b), positive control showing gK-UL20p interaction (c), wild type (d), UL20Δ6 (e), UL20ΔPhe (f), UL20F222A (g), UL20F210A (h), and UL20F205-206A (i). DAPI-stained nuclei are shown in blue, and PLA-positive signals generated by the Texas Red dye are shown in red.