TABLE 2.
Determination of HSV-1 genome-to-PFU ratios
| Virus | Total no. of genomes/PFUa |
|---|---|
| Wild type | 48,000 |
| UL20Δ6 | 538,000 |
| UL20ΔPhe | 170,000 |
| UL20F222A | 166,000 |
| UL20F210A | 38,000 |
| UL20F205-206A | 5,648,000 |
a
The total number of viral genomes in infected Vero cells at 24 h.p.i. was obtained by qPCR. The total number of intracellular infectious virions was obtained by plaque assay on FRT cells. Ratios reflecting relative efficiency of envelopment and infectious virion production were obtained by dividing the average of the number of viral genomes within the infected cells by the number of PFU.