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The nuclear export of BM1 depended on NES1 and NES2. (A) The NES1 and/or NES2 were mutated in the context of full-length EGFP tagged BM1. (B) The constructs encoding the WT or mutant EGFP-BM1 were transfected into 293T cells, respectively. At 20 h posttransfection, the cells were fixed PBS (4% paraformaldehyde) and stained with DAPI. The intracellular localization of the indicated proteins was imaged by confocal laser scanning fluorescence microscopy (Olympus LSCMFV500).
