FIG 5.

NLS1 mediated the nuclear import of BM1. (A) The critical residues in NLS1 were substituted by alanine in the context of full-length EGFP tagged BM1. (B) The constructs encoding EGFP, the WT and mutant EGFP-BM1 were transfected into 293T cells, respectively. At 20 h posttransfection, the cells were treated with (b, d, f, and h) or without (a, c, e, and g) LMB (11 nM). After a 3-h incubation, the cells were fixed PBS (4% paraformaldehyde) and stained with DAPI. The intracellular localization of the indicated proteins was imaged by confocal laser scanning fluorescence microscopy (Olympus LSCMFV500).