FIG 7.

Identification of the phosphorylated residues in BM1 by MS. (A) Cell lysates of mock- or influenza B virus-infected MDCK cells were treated with or without ALP, immune precipitated with or without monoclonal anti-BM1 antibody, and subjected to gel electrophoresis. The band of candidate phosphorylated BM1 was collected from the gel and analyzed by LC-MS/MS. The candidate band was identified as BM1, and two peptides detected using MS (B and D) revealed the phosphorylated residues. (C) The peptides detected using MS (blue) and phosphorylated residues (red) are indicated in the context of BM1.