FIG 9.

Role of phosphorylated S80 and T84 in the nuclear import of BM1. 293T cells were transfected with constructs encoding EGFP-tagged full-length BM1 with alanine substitution of S80 and T84. At 20 h posttransfection, LMB was added to the culture medium of the indicated cells to the concentration of 11 nM. After a 3-h incubation, the cells were fixed PBS (4% paraformaldehyde) and stained with DAPI. The intracellular localization of the EGFP-BM1–S80/T84A was imaged by confocal laser scanning fluorescence microscopy (LSCM Leica SP8). The three-dimensional images were created with the corresponding two-dimensional images taken along the Z-axis (software Imaris).