FIG 2.

GII.4 mouse and human MAb blockade responses against chronic-infection strains. (A to I) Mouse and human GII.4 monoclonal antibodies were assayed for the ability to block GII.4-2006b, P.D1, and P.D302 VLP interaction with carbohydrate ligand. The mean percent control binding (the percentage of the VLP bound to carbohydrate ligand in the presence of an antibody compared to the amount of VLP bound with no antibody present) of each VLP was fitted with a sigmoidal curve, and the mean EC₅₀ (μg/ml) blockade titers for GII.4-2006b, P.D1, and P.D302 were calculated. The error bars represent 95% confidence intervals. The asterisks indicate that the mean EC₅₀ blockade titer for the test VLP was significantly different from the mean EC₅₀ for GII.4-2006b (P < 0.05) (*) or was significantly different from those of both GII.4-2006b and P.D1 (P < 0.05) (**). Monoclonal antibodies that did not block a particular VLP were assigned an EC₅₀ of 2 times the upper limit of detection for statistical analysis and are shown on the graph as data points above the upper limit of detection (dashed lines). Statistics were calculated by one-way ANOVA with a Bonferroni posttest.