FIG 4.

Evaluation of the nuclear export activity of SVLT-NEP fusion proteins. (A) Schematic representation of the SVLT-NEP chimeric protein. (B) 293T cells were transfected with plasmids encoding SVLT, SVLT-NEP (WSN), or SVLT-NEP (CA04). At 24 h posttransfection, the cells were treated with 100 ng/μl cycloheximide for 3 h to block protein synthesis. LMB (11 nM) was added to the medium along with the cycloheximide for LMB treatment. (C) A Western blot assay of 293T cells transfected with FLAG-SVLT or SVLT-NEP fusion protein constructs was carried out at 24 h posttransfection. An anti-FLAG monoclonal antibody was used to detect the target proteins.