Fig. 2.

A real example of the effects of the missing wedge. The image shows a cross-section from a reconstruction of a frozen Escherichia coli cell, with a gold fiducial marker visible at the extreme left. The cell membranes are only resolved at the sides of the cell, and white streaking artefacts are visible around the gold particle. The black lines show tangents to the outer cell membrane and their angles from the z axis. The angular range of the tilt series used for this tomogram is shown above the image.