Day 3 total (net) CD1d reactivity was measured as described previously (
19,
21,
22,
33,
48,
49). Limit of detection was 1 ng.mL
−1; modest responses were 1–10 ng.mL
−1; strong responses: >100 ng.mL
−1. Pure blood iNKT lines provided positive controls.
Ex vivo CD1d-specific and mitogen IFNγ responses of IHL from HCV± subjects, compared to in vitro-expanded IHL.
% CD1d-specific IFNγ responses of IHL ex vivo relative to mitogen, compared to HCV± donor in vitro-expanded IHL. There were no significant IHL responses to invariant NKT-specific ligand αGalcer (not shown), although pure blood iNKT served as positive controls.
Total (net) CD1d-specific and mitogen IFNγ responses of IHL from subjects without HCV were assayed ex vivo compared to matched donor in vitro-expanded IHL and pure iNKT controls.
CD1d-specific and mitogen IL-4 responses of the same matched IHL as in 1C. No CD1d-specific IL-4 was detected from these IHL.
CD1d-specific and mitogen IL-10 responses of the same matched IHL as in 1C.