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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Viral Hepat. 2013 Mar 11;20(8):556–565. doi: 10.1111/jvh.12081

Figure 1. Comparison of hepatic CD1d-reactive T cells assayed directly ex vivo versus after in vitro expansion: cytokine profile of hepatic CD1d-reactive T cells ex vivo.

Figure 1

Day 3 total (net) CD1d reactivity was measured as described previously (19,21,22,33,48,49). Limit of detection was 1 ng.mL−1; modest responses were 1–10 ng.mL−1; strong responses: >100 ng.mL−1. Pure blood iNKT lines provided positive controls.
  1. Ex vivo CD1d-specific and mitogen IFNγ responses of IHL from HCV± subjects, compared to in vitro-expanded IHL.
  2. % CD1d-specific IFNγ responses of IHL ex vivo relative to mitogen, compared to HCV± donor in vitro-expanded IHL. There were no significant IHL responses to invariant NKT-specific ligand αGalcer (not shown), although pure blood iNKT served as positive controls.
  3. Total (net) CD1d-specific and mitogen IFNγ responses of IHL from subjects without HCV were assayed ex vivo compared to matched donor in vitro-expanded IHL and pure iNKT controls.
  4. CD1d-specific and mitogen IL-4 responses of the same matched IHL as in 1C. No CD1d-specific IL-4 was detected from these IHL.
  5. CD1d-specific and mitogen IL-10 responses of the same matched IHL as in 1C.