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Binding curves of fluorescein-tagged LD4 peptides titrated with increasing concentrations of FAT-LD2, as measured by fluorescence anisotropy in 10-mM phosphate, pH 8.0. The y-axis indicates the fraction of maximal binding. a-c LD4, LD4-S273p, and LD4-S275p, respectively. d LD4 in 15% TFE.