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. 2014 Jun 12;5:222. doi: 10.3389/fphys.2014.00222

Figure 1.

Figure 1

Cancer hallmark transformation phenotypes of SWCNT-exposed MeT-5A and LP-9 cells. Anchorage-independent cell growth was determined by soft agar colony formation assay. Two-month exposed MeT-5A and LP-9 cells in culture medium containing 0.33% agar were plated onto the dish coated with 0.5% agar in culture medium. Colonies were examined by light microscopy after 4 weeks of incubation (A). Invasion was assessed in the SWCNT-exposed cells using BD Matrigel® invasion chamber. Invading cells were fixed, stained and visualized under a microscope. The number of invading cells was counted and presented as a bar chart (B). *Significantly difference from control with P < 0.05 (n = 3).