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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: J Cell Sci. 2014 Jan 14;127(0 5):1052–1064. doi: 10.1242/jcs.141226

Figure 4. Podosome-like protrusive structures contain MMP14 and tubulin.

Figure 4

(A) Confocal images of dendritic cells cultured on filters with 1 μm pore sizes that were impregnated with double-quenched FITC-labeled collagen in gelatin (DQ collagen; green). Actin was stained with phalloidin-Alexa fluor 633 (Phal; magenta). Collagen degradation results in loss of self-quenching of the FITC fluorophore and an increase in fluorescence. (B) Fluorescence intensity profiles from panel A marked by the dashed lines. (C) Confocal images (left) and quantification (right) of dendritic cells cultured on glass and membrane filters with pore sizes of 1 μm. Actin was stained with phalloidin-Alexa fluor 546 (magenta) and the metalloprotease MMP-14 was labeled by specific monoclonal antibodies and secondary antibodies conjugated to Alexa fluor 488 (AB; green). The yellow line indicates the positions of the orthogonal view. The yellow arrow heads indicate randomly chosen actin cores. The red arrow head indicates the approximate surface of the filter. (D) Same as panel C, but now for tubulin. Error bars show the spread of data for multiple cells from at least two independent experiments. Scale bars, 5 μm.