Table 7.
Rate of evolution of ESE and (a) non-ESE sequence and (b) pseudoESE sequence at four-fold degenerate sites
| Dataset | Median ESE | Median non-ESE | % Difference | P two-sided |
|---|---|---|---|---|
| A | ||||
| ESR |
0.3 |
0.39 |
-23.1 |
1.10E-13 |
| INT2.400 |
0.32 |
0.36 |
-11.1 |
1.10E-13 |
| INT3 |
0.3 |
0.36 |
-16.7 |
1.10E-13 |
| INT3.400 |
0.3 |
0.35 |
-14.2 |
1.10E-13 |
| INT3_ESR |
0.3 |
0.35 |
-14.2 |
1.10E-13 |
| INT3_ESR_400 |
0.29 |
0.35 |
-17.1 |
3.40E-13 |
| Ke-ESE |
0.35 |
0.34 |
2.9 |
8.20E-08 |
| Ke-ESE400 |
0.38 |
0.34 |
11.8 |
1.10E-13 |
| PESE |
0.31 |
0.37 |
-16 |
1.10E-13 |
| RESCUE |
0.32 |
0.35 |
-8.5 |
5.70E-13 |
| B |
|
|
|
|
| ESR |
0.3 |
0.35 |
-14.3 |
4.50E-13 |
| INT2.400 |
0.32 |
0.35 |
-8.5 |
4.50E-13 |
| INT3 |
0.3 |
0.35 |
-14.3 |
4.50E-13 |
| INT3.400 |
0.3 |
0.34 |
-11.8 |
4.50E-13 |
| INT3_ESR |
0.3 |
0.34 |
-11.8 |
4.50E-13 |
| INT3_ESR_400 |
0.29 |
0.34 |
-14.7 |
2.30E-12 |
| Ke-ESE |
0.35 |
0.35 |
0 |
4.10E-06 |
| Ke-ESE400 |
0.38 |
0.36 |
5.6 |
4.50E-13 |
| PESE |
0.31 |
0.35 |
-11.4 |
4.50E-13 |
| RESCUE | 0.32 | 0.35 | -8.5 | 4.50E-13 |
We consider the proportion of four fold degenerate sites that are changed or unchanged when comparing mouse-human alignments. We split sites by whether in human the sequence matches an ESE or not (Table A) or ESE versus pseudoESE (Table B). We then perform a Wilcoxon paired test considering the rate of evolution of ESE and non-ESE or pseudoESE at each position away from an exon boundary. We also present the % difference between the medians, this being (median for ESE - median for non-ESE)/median for non-ESE. All tests are significant after Bonferonni correction (for consistency the P values are shown in bold).