Fig. 1.

Linear diagram and SDS-PAGE analysis of mature rat apoA-IV and recombinant deletion mutants. A: The sequence is numbered according to the mature apoA-IV sequence. The white boxes represent putative 22 amino acid amphipathic alpha helices. Most of the repeats are punctuated by proline residues (P). The arrows are placed at sites where deletions were made either in the C- or N-terminus. Each deletion mutant used in this study is represented by a solid black line showing the sequence that was left intact. B: After expression and purification as described in Experimental Procedures, 4 μg samples of apolipoprotein were electrophoresed on an 18% denaturing polyacrylamide gel and stained with Coomassie blue. Lane 1: Amersham low molecular weight protein standards; Lane 2: apoA-IV Δ249–371; Lane3: apoA-IV Δ117–371; Lane 4: WT rat apoA-IV; Lane 5: standards; Lane 6: Δ1–61.