Fig. 2.

Food intake measurements in i3vt cannulated rats. A: 24 h fasted rats were injected with either saline alone or the indicated amount of apoA-IV (recombinant or native rat apoA-IV) immediately before dark-onset and accumulated food intake was measured up to 4 h. *P < 0.05, **P < 0.01 vs. Saline, n = 5. B: Accumulated food intake was plotted as a function of time post injection of 4 μg recombinant apoA-IV. LPS (+) refers to a preparation of recombinant apoA-IV as derived from the His-binding column described in Experimental Procedures prior to injection into the 3rd ventricle of the rat brain. LPS (−) refers to a similar sample that had been passed down an endotoxin affinity column, **P < 0.01 vs. Saline, n = 5.