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Preformed DMTAP-ND or DMPC-ND were mixed with dsOligo, electrophoresed on an agarose gel (1% w/v) and stained with ethidium bromide. DMTAP-ND and dsOligo were incubated at given charge ratios achieved by adjusting the amount of DMTAP-ND added to a fixed amount of dsOligo.
