Figure 5.

Survival of Escherichia coli deletion mutants for genes in which codon adaptation was linked to thermophilicity or halophilicity. Survival of strains with deletions in (A) putative heat stress and (B) putative osmotic stress genes discovered through differential codon adaptation in thermophilic or halophilic microbes (at false discovery rate (FDR) ≤6.6% and ≤33.9% respectively). Error bars show the 95% CI of the mean, determined over at least four replicates. Strains are ordered by survival, normalized to the wild-type survival under the same stress (23.6% for the heat and 21.3% for the osmotic shock after normalization, both shown as 100% on the plots). The dnaJ and otsA strains were included as a positive control.