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. 2014 Feb 25;15(2):R27. doi: 10.1186/gb-2014-15-2-r27

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Copyright © 2014 Riveron et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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GSTe2 purification and X-ray three-dimensional structure. (A) Size-exclusion chromatogram of GSTe2 alleles from Benin (BN), Uganda (UG) and Malawi (MAL) alleles. The lines show the absorbance recorded at 280 nm. Molecular-weight markers (Bio-Rad, Hercules, CA, US) are indicated in kilodaltons. (B) SDS-PAGE gel of the three purified GSTe2 alleles (26.8 kDa). (C) Topology of GSTe2 showing the C- and N-terminals, the GSH binding pocket (G-site) and the substrate-binding pocket (H-site). AU, arbitrary units; BN, Benin; GSH, glutathione; MAL, Malawi; MW, molecular weight; UG, Uganda; vol, volume.