Figure 6.

AS events in PpHYH2 transcripts. (A) Revised gene models of the PpHYH2 locus. Expressed exonic regions (black) and putative isoforms are shown. Asterisks over each isoform indicate the start codons, and triangles represent the stop codons. Polypeptides encoded by the alternatively spliced isoforms were shown on the right. Sizes of the polypeptides are shown in the C-terminal end. Gray box, bZIP DNA-binding domain. (B) qRT-PCR analysis of relative expression of PpHYH2 IR isoforms in pooled RNA from dark-grown (D), 1-h light-treated (R1 and B1), 4-h light-treated (R4 and B4) samples tested in triplicate with primer sets designed for PpHYH2 IR isoforms, total transcripts of the corresponding genes, and PpACT2 (see Additional file 7).