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. 2013 Dec 11;4(6):148. doi: 10.1186/scrt359

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Copyright © 2013 Niada et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Osteogenic potential of pASCs. Quantification of collagen (A), calcified extracellular matrix (ECM) deposition (C), alkaline phosphatase (ALP) activity (E) in undifferentiated (CTRL, white bars), and osteo-differentiated (OSTEO, dark bars) ScI- and BFP-pASCs; data are expressed as mean ± SEM (n = 12). OSTEO versus CTRL *P < 0.05; **P < 0.01; ***P < 0.001. Images of ScI- and BFP-pASC wells stained with Sirius Red (B, left panel) and Alizarin Red-S (D, left panel) and representative microphotographs of ScI-pASCs (B, D, right panel, optical microscopy, 40× magnification; scale bar, 200 μm). Osteonectin expression of ScI-pASCs and BFP-pASCs analyzed with Western blot; its quantification, normalized to β-actin, is also indicated (F).