Figure 2.

Generation of human glutathione S-transferase Mu 2 mesenchymal stem cell (hGSTM2-MSC) cell line and detection of hGSTM2 expression. (A) Strategy of cloning the hGSTM2 gene into lentiviral vector pCDH-MSCV-MCS-EF1-GFP-Puro. (B)In vitro cultured MSCs before transduction. (C) MSCs transduced with pCDH-hGSTM2. (D, E) Detection of hGSTM2 expression by (D) reverse transcription quantitative PCR and (E) Western blotting. hGSTM2 expression was found only in MSCs transduced with pCDH-hGSTM2 and not in MSCs transduced with pCDH vector alone.