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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Epigenomics. 2013 Aug;5(4):439–452. doi: 10.2217/epi.13.37

Figure 2. Proteomics of isolated chromatin.

Figure 2

After cell lysis and restriction digestion, the targeted loci are captured by primers that bind to a specific genomic region. An enzymatic step incorporates biotin labels only to chromosomal fragments that contain the targeted sequence and streptavidin-coated magnetic particles isolate the targeted chromatin. Proteins associated with the isolated regions are separated by SDS-PAGE and then analyzed by MS.

LC–MS: Liquid chromotography–mass spectrometry; MS: Mass spectrometry.