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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Nat Commun. 2013;4:1565. doi: 10.1038/ncomms2582

Figure 3. macroH2A2 is the predominant epigenetic barrier in reprogramming.

Figure 3

(a) Chromatin extracts from wt, macroH2A1 -/- (m1KO), macroH2A2 -/- (m2KO) and dKO DFs probed with macroH2A1 and macroH2A2 antibodies. Amido Black of core histones used for loading. (b) Representative wells of AP positive iPS colonies at 12 days post-infection, bright field image of single colonies shown; Scale bar, 100μm. Quantitation of fold-change in AP positive colonies over wt (shown right); mean ± s.d. (n=4); p value (asterisk): p=0.002 (wt vs dKO). (c) Chromatin extracts from dKO DFs expressing GFP-tagged macroH2A isoforms (top arrow), H2B-GFP (bottom arrow) and vector alone blotted for GFP. Amido Black of core histones used for loading. (d) Representative wells of AP positive iPS colonies at 12 days post-infection, bright field and fluorescence image of individual dKO colonies expressing H2B-GFP and macroH2A GFP-tagged isoforms; Scale bar, 100μm. Quantitation of fold-change in AP positive colonies over wt (shown right); [o] represents uninfected; mean ± s.d. (n=3); unpaired Student's test (two tailed) p value (asterisk): p=0.007 (wt vs dKO+H2B); p=0.036 (dKO+H2B vs dKO+m2).