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. 2014 Apr 8;5(2):47. doi: 10.1186/scrt435

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Copyright © 2014 Kadari et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Improved cardiac differentiation potential of transgene-free induced pluripotent stem cells. (A) Genomic polymerase chain reaction (PCR) for the confirmation of transgene deletion. fl-ARiPS clone 1 was treated with 2 μM TAT-Cre for 5 hours and PCR was performed using primers against WPRE and β-actin (BA). (B) Cardiomyocyte differentiation of iPSCs. fl-ARiPS clone 1 and its TAT-Cre-treated polyclonal cell progeny were differentiated towards cardiomyocyte lineage using cardio-inductive medium. Cells were stained with α-actinin antibody at day 15. Scale bar: 40 μm. (C) Flow cytometry analysis of cardiac-specific troponin T (cTNT) staining at day 15 of cardiac differentiation. Cardiac differentiation showed an increase from 37 to 56% cTNT-positive cardiomyocytes in the case of TAT-Cre-treated cell populations. The experiment was repeated twice with similar results.