Figure 7.

Western blot and mRNA analysis of vimentin in the descending aortas after artificial shear stress. Vimentin expression in 12-month-old wild type (WT) and mLMNA⁺ descending aortas provided control values for a study that involved a 30-minute ex vivo exposure to high fluidic shear stress. In triplicate samples no difference was detected between WT and mLMNA⁺ control samples not exposed to shear stress (A). This image was the same figure as Figure 6A (12 m descending WT and mLMNA⁺). After exposure to shear stress, a 50% reduction in vimentin expression was demonstrated in mLMNA⁺ mice compared with WT mice (A). Vimentin mRNA expression in descending aortas of WT and mLMNA⁺ mice was obtained by RT-PCR analysis after exposure to shear stress (B).