Table 1.
PCR primers used to produce three overlapping segments of CA XII.
| Segment number | Segment sequence | PCR primer sequences* | Restriction endonuclease |
|---|---|---|---|
| #1 | Val 27-Gly130 | 5′ CCCGGGATCCGTGAACGGTTCCAAG 3′ | BamHI |
| 5′ ACGGCGGCCGCTTAGCCGTGCGGGTCATT 3′ | NotI | ||
|
| |||
| #2 | Ser111-Leu210 | 5′ GGGCGGATCCTCTCGCTACAGTGCC 3′ | BamHI |
| 5′ GTCCTCTCGAGTTACAGCTCTTCAATGTTG 3′ | XhoI | ||
|
| |||
| #3 | Asp184-Ser290 | 5′ TCCGGGATCCGACAAGATCTTCAGTC 3′ | BamHI |
| 5′ AGACCTCGAGTTAGGAGAAGGAGGTGTATAC 3′ | XhoI | ||
*Restriction endonuclease recognition sites are underlined.