Table 2.
Quantification of cell surface markers by flow cytometry
| Marker | BM-MSC | AT-MSC | WJ-MSC |
|---|---|---|---|
|
CD73 |
99.0 ± 0.9 (65.3 ± 8.6) |
92.3 ± 7.0 (153.3 ± 95.4) |
96.4 ± 3.3 (283.7 ± 259.6) |
|
CD90 |
98.3 ± 0.8 (125.9 ± 63.6) |
97.0 ± 3.1 (203.6 ± 94.7) |
99.5 ± 0.4 (295.4 ± 79.2) |
|
CD105 |
94.3 ± 7.3 (66.0 ± 44.3) |
94.3 ± 5.7 (142.4 ± 78.8) |
87.4 ± 11.7 (151.3 ± 196.0) |
|
CD45 |
0.8 ± 0.9 (3.5 ± 1.6) |
0.6 ± 0.7 (11.3 ± 11.0) |
1.1 ± 1.1 (7.9 ± 3.8) |
|
CD14 |
1.5 ± 0.8 (2.6 ± 0.8) |
0.8 ± 1.0 (13.6 ± 0.1) |
0.9 ± 0.7 (8.3 ± 4.4) |
|
CD34 |
0.7 ± 0.6 (2.8 ± 1.6) |
0.7 ± 0.9 (2.6 ± 2.1) |
2.6 ± 2.7 (8.7 ± 4.7) |
|
CD31 |
0.9 ± 1.1 (10.1 ± 7.9) |
0.3 ± 0.5 (3.0 ± 1.0) |
1.3 ± 2.0 (5.9 ± 5.5) |
|
CD44 |
99.6 ± 0.2 (233.2 ± 58.1) |
99.3 ± 0.1 (1,036.0 ± 1,306.4) |
95.6 ± 5.4 (231.7 ± 118.3) |
|
CD54 |
40.0 ± 11.5 (6.0 ± 1.9) |
4.6 ± 3.7 (5.7 ± 4.8) |
97.2 ± 1.7 (81.3 ± 94.7) |
|
CD146 |
81.7 ± 4.4 (26.5 ± 24.4) |
24.9 ± 3.4 (38.9 ± 10.6) |
90.9 ± 6.0 (110.4 ± 48.4) |
|
CD166 |
94.7 ± 7.8 (71.2 ± 17.8) |
72.4 ± 29.9 (143.2 ± 59.1) |
91.0 ± 3.6 (118.9 ± 8.8) |
|
HLA-DR |
1.8 ± 2.4 (6.0 ± 6.3) |
1.1 ± 1.2 (29.1 ± 17.9) |
1.1 ± 1.1 (8.0 ± 3.2) |
| IgG | 0.7 (8.24) | 0.6 ± 0.0 (7.3 ± 5.1) | 0.6 (22.8) |
Results are expressed in mean percentage ± standard deviation, with MPI values in brackets. AT-MSC: adipose tissue-derived mesenchymal stromal cells, BM-MSC: bone marrow-derived mesenchymal stromal cells, WJ-MSC: Wharton’s Jelly-derived mesenchymal stromal cells.