Table 1.
Percentage expression of phenotypic cell markers in cbMSCs, cmMSCs, and cbMNCs
| Marker | cbMSC | cmMSC | cbMNC | Function of marker |
|---|---|---|---|---|
| CD7 |
0.407 |
5.4 |
62.51 |
Marker for thymocytes and mature T cells |
| CD4 |
5.88 |
68.0 |
26.77 |
Marker for T-helper cells, regulatory T cells, monocytes |
| CD25 |
91.2 |
71.3 |
71.8 |
Part of IL-2 receptor present on activated T cells/B cells |
| CD3 |
36.3 |
4.33 |
31.3 |
T-cell coreceptor and marker for mature T cells |
| CD8 |
0.123 |
0.277 |
21.1 |
Coreceptor for T-cell receptor and binds specifically to major histocompatibility complex (MHC) I |
| CD44 |
100 |
99.7 |
81.7 |
Marker for effector memory T cells, lymphocyte activation, receptor for hyaluronic acid |
| HLA-ABC |
96.9 |
98.8 |
92.4 |
MHC Class I |
| CD90 |
73.5 |
99.8 |
36.5 |
Marker for mesenchymal stem cells, hematopoietic stem cells (HSCs), and fibroblasts |
| CD56 |
1.28 |
80.8 |
11.3 |
Neuronal cell-adhesion molecule, plays role in synaptic plasticity and neurite outgrowth |
| CD210 |
91.8 |
96.5 |
0.544 |
IL-10 receptor on T cells/B cells, natural killer (NK) cells, and monocytes |
| CD45 |
0.99 |
0.82 |
87.7 |
Leukocyte common antigen |
| CD14 |
0.404 |
20.3 |
12.4 |
Monocyte differentiation antigen |
| CD34 |
0.124 |
1.57 |
0.80 |
Marker for primitive hematopoietic and endothelial stem cells |
| CD133 |
0.11 |
0.899 |
0.897 |
Prominin-1, specific for hematopoietic stem cells, endothelial progenitors, neuronal and glial stem cells |
| CD117 |
99.5 |
92.9 |
2.2 |
Stem cell growth-factor receptor, expressed on HSCs, multipotent progenitors, common myeloid progenitors |
| CD73 |
100 |
99.5 |
5.75 |
Lymphocyte differentiation marker |
| CD123 |
91.8 |
48.8 |
1.81 |
IL-3 receptor on pluripotent progenitors |
| CD16 |
31.5 |
72 |
81.9 |
Marker for NK cells, monocytes, and macrophages |
| CD166 |
100 |
99.8 |
3.38 |
Activated leukocyte cell-adhesion molecule |
| Lin1 |
0.46 |
31.6 |
87.2 |
Lineage cocktail 1 (CD3, 14, 16, 19, 20, 56) |
| CD59 |
99.8 |
99.3 |
7.14 |
Complement regulatory protein, regulates complement-mediated cell lysis by formation of membrane-attack complex |
| CD106 |
16.21 |
23.0 |
3.54 |
Vascular cell-adhesion molecule (VCAM)-1 |
| CD184 |
100 |
75.6 |
64.4 |
Chemokine (C-X-C motif) receptor-4 specific for stromal-derived factor-1 |
| HLA-DR |
0.434 |
0.312 |
31.6 |
MHC Class II |
| CD19 |
6.89 |
0.399 |
9.27 |
Marker for follicular dendritic cells and B cells |
| CD33 | 1.26 | 0.313 | 27.3 | Marker for myeloid-lineage cells |
Represents comparative percentage expression of various markers assessed with flow cytometry by using Becton Dickinson LSR II (Stanford Shared FACS facility).