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NK degranulation assay. Surface expression of CD107a in resting (A) or pre-activated (B) PBMCs. Three culture conditions were analyzed: PBMCs alone (negative control), PBMCs with the addition of K562 (positive control) and PBMCs with MSCs. For this flow cytometry assay, CD3- CD56+ NK cells were gated. MSCs, mesenchymal stem cells; NK, natural killer, PBMCs, peripheral blood mononuclear cells.
