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. 2014 May 27;171(12):2993–3009. doi: 10.1111/bph.12680

Figure 3.

Figure 3

LPS induces VCAM-1 expression via c-Src-dependent MAPKs activation. (A) RASFs were pretreated with U0126, SB202190 or SP600125 for 1 h and then incubated with LPS for 6 h. (B) Cells were transfected with empty vector (pTOPO-U6) or with the indicated shRNA and then incubated with LPS for 6 h. (C) RASFs were pretreated with U0126 (U0; 10 μM), SB202190 (SB; 10 μM) or SP600125 (SP; 10 μM) and then incubated with LPS for the indicated time intervals. (D) Cells were transfected with an empty vector or c-Src shRNA and then incubated with LPS for the indicated time intervals. The levels of various proteins were analysed by Western blot using the antibodies indicated. All analyses were performed on samples from three RA patients. Results are representative of three independent experiments. Values (A–D) are the mean ± SEM. *P ≤ 0.05; #P ≤ 0.01 versus LPS alone.