Table 5.
Quality of assays: signal window
| Native human islets | Human re-aggregated clusters | |
|---|---|---|
| Bay K 8644 | −0.25 | 3.89* |
| Glibenclamide | −7.67 | 5.40* |
| Tolbutamide | 0.18 | 2.19* |
| Carbachol | −6.5 | 1.67 |
| Caffeine | −3.54 | 0.73 |
| GLP-1 | −29.01 | 0.29 |
| IBMX | −3.3 | 6.68* |
| Nifedipine | −4.07 | 6.78* |
The signal window is a based on the positive and negative controls used for each plate. In this case the negative control was 11.2 mM glucose with no added drug. The positive control was 20 mM glucose + 30 mM K+.
*
Signal windows >2 are rated as excellent (Iversen et al., 2004)