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. 2014 May 20;2014:235426. doi: 10.1155/2014/235426

Figure 4.

Figure 4

Downregulation of NF-κB pathway in muscle over-expressing V1aR. (a) Western blot analysis revealed that TNF overexpression promotes downregulation of IκBα with concomitant upregulation of the phosphorylated form of NFκB (p-NF-κB-p65) expression. By contrast, p-NF-κB-p65 expression was significantly reduced in muscle overexpressing TNF and V1aR together compared with TNF alone. The expression of native NF-κB-p65 does not reveal significant differences between the various samples. (b, c) Densitometric analysis of two independent experiments for phospho-NF-κB-p65 versus native NF-κB-p65 (b) and IκBα versus GAPDH (c) expression. ∗# P < 0.05; **P < 0.01 by Student's t-test.