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. 2014 Jun 15;25(12):1845–1853. doi: 10.1091/mbc.E13-08-0450

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© 2014 Murrell and Gardel. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Reconstituted cell cortex in vitro. (A) Schematic of the experimental system. F-actin is crowded down to the surface of a multicomponent phospholipid bilayer via methylcellulose, after which myosin II motors and cross-linkers are added. (B) F-actin network (red) decorated with thick filaments of skeletal muscle myosin II (green; ρ = 0.07 μm⁻²). Bulk concentration of F-actin is 1.3 μM (R_xlink = 0, R_adh = 0).