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. 2014 Jun 15;25(12):1867–1876. doi: 10.1091/mbc.E14-03-0839

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© 2014 Vaughan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Blocking generation of DAG, but not PA, inhibits Rho and Cdc42 activation. (A) Cells were injected with eGFP-rGBD and mRFP-wGBD to detect active Rho and active Cdc42, respectively. In cells treated with D609, Rho and Cdc42 activity is inhibited and wound healing stalls, whereas FIPI treatment does not inhibit GTPase activity or healing. (B) Intensity of Rho and Cdc42 zones from control and D609-treated cells was quantified at 60 s (Rho: control vs. D609, p < 0.01; Cdc42: control vs. D609, p < 0.005). (C) Intensity of Rho and Cdc42 zones from control and FIPI-treated cells was quantified at 60 s (Rho: control vs. FIPI, p >0.05; Cdc42: control vs. FIPI, p < 0.01). Top and bottom whiskers represent maximum and minimum values, respectively. Scale bar, 20 μm.