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. 2014 Jun 15;25(12):1867–1876. doi: 10.1091/mbc.E14-03-0839

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© 2014 Vaughan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Dose-dependent suppression of healing and DAG production by SPK601 or D609. (A) Cells were treated with DMSO, the vehicle, or the indicated micromolar concentrations of SPK601 (S) or D609 (D) for 1 h, stab wounded with a glass needle, cultured overnight, and then assessed for survival. SPK601 and D609 both significantly suppressed healing at concentrations of 100 or 200 μM. (B) Quantification of wound DAG levels in cells treated as in A. Bars, mean ± SD; *p < 0.05; ***p < 0.005.