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. 2014 Jun 15;25(12):1867–1876. doi: 10.1091/mbc.E14-03-0839

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© 2014 Vaughan et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — PKCβ and PKCη differentially regulate Rho and Cdc42 zones. (A) Cells injected with PKCβ -eGFP and PKCη-mCherry reveal localization of both PKCs to the wound edge, with PKCβ spanning a broader region than PKCη. (B) A line scan from the 90-s time point of A. (C) Cells were injected with eGFP-rGBD and mRFP-wGBD alone or with PKCβ, DN PKCβ, PKCη, DN PKCη, or DN PKCβ and PKCη. PKCβ up-regulates Rho and Cdc42 activity zones, whereas DN PKCβ down-regulates zones. PKCη inhibits Rho and Cdc42 activity zones, whereas DN PKCβ up-regulates Rho and Cdc42 activity on the cortex. Expression of DN PKCβ and full-length PKCη results in dramatic inhibition of both Rho and Cdc42 activity zones. (D) Quantification of Rho and Cdc42 zone intensity at 30 and 60 s with PKCβ expression (control, n = 6; PKCβ, n = 7). (E) Quantification of Rho and Cdc42 zone intensity at 60 s and background intensity with DN PKCβ expression (control, n = 16; PKCβ DN, n = 11). (F) Quantification of Rho and Cdc42 zone intensity at 30 and 60 s with PKCη expression (n = 14). (G) Quantification of Rho and Cdc42 zone intensity at 60 s and background intensity with DN PKCη expression (control, n = 16; PKCη DN, n = 11). (H) Quantification of Rho and Cdc42 zone intensity at 30 and 60 s with expression of DN PKCβ and full-length PKCη (control, n = 16, PKCβ DN/PKCη, n = 13). Green bars, Rho; red bars, Cdc42. Top and bottom whiskers represent maximum and minimum values, respectively. Scale bar, 20 μm. W, wound.