FIGURE 6:
Live-cell imaging of HeLa cells cotransfected with mCherry-tagged stx8 and EGFP-tagged hTASK-1. (A–G) Cotransfection of TASK-1 and stx8; the Pearson coefficient was 0.89 ± 0.02 (n = 7 cotransfection experiments, 37 cells). Plain arrows indicate colocalization; crossed arrows indicate lack of colocalization. (D–F) Higher magnifications of the region indicated by the square in C. (H–N) Cotransfection of TASK-1 and stx8Q179A; the Pearson coefficient was 0.86 ± 0.02 (n = 4 cotransfection experiments, 33 cells). (O–Q) Cotransfection of TASK-1 with stx8Δ216-233; the Pearson coefficient was 0.41 ± 0.03 (n = 4 transfection experiments, 33 cells). (K–M) Higher magnifications of the regions indicated by the square in J. All images were taken 48 h after transfection. Scale bars, 5 μm (A–C, G–I), 1 μm (D–F, K–M). (G, N) Intensity profiles of F and M (green line, EGFP; red line, mCherry). For calculating the Pearson coefficient, the entire cell was selected as region of interest.