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. 2014 Jun 15;25(12):1877–1891. doi: 10.1091/mbc.E13-10-0592

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© 2014 Renigunta et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — TIRF microscopy of TASK-1 and stx8. (A, B) HeLa cells transfected with EGFP-tagged hTASK-1 and mCherry-tagged stx8; scale bars, 5 μm. (C) Sequence of TIRF images taken from the cell shown in A; scale bars, 0.5 μm. The two bottom rows show the red and green channels (Materials and Methods). TASK-1 and stx8 are colocalized in diffraction-limited spots for at least 37 s. (D) Sequence of TIRF images taken from the cell shown in B; scale bars, 0.5 μm. The two bottom rows show the red and green channels (Materials and Methods). TASK-1 and stx8 are colocalized in diffraction-limited spots for at least 4.6 s.