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. 2014 Apr 2;33(6):1491–1497. doi: 10.3892/ijmm.2014.1715

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Western blot analysis was used to investigate the effects of rapamycin on components of the mammalian target of rapamycin (mTOR) pathway. (A) Phospho-p70S6 kinase (p70S6K) expression levels were decreased following treatment with rapamycin in a dose-dependent manner. (B) Analysis of the effects of treatment with rapamycin and/or U0126. Treatment with rapamycin resulted in a dose-dependent increase in LC-3II and Atg12-Atg5 complex expression. On the contrary, p62/SQSTM1 expression was decreased in a dose-dependent manner. Cleaved PARP and cleaved caspase-3 expression was increased by rapamycin and rapamaycin-plus-U0126 treatment. Rap, rapamycin.