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Fluorescence microscopy images. (A) The expression of pEGFP-LC3 was used to evaluate autophagy. pEGFP-LC3 dot formation was significantly increased by rapamycin treatment. (B) Apoptotic cells were detected by Annexin V and PI staining. There were more Annexin V-stained cells in the group treated with both rapamycin and U0126 than in the other groups. Rap, rapamycin.
